FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013564

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013564
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1156091
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTAGA5750.002788674310.0696771
CCCAGAT7953.8315964E-510.0142621
TCACAAC7200.00178633059.0199692
CCTTAAT6550.0081606248.8397941
CCCATGG6550.0081606248.8397941
CCCAGAA11051.6929112E-58.5148011
CTTTAAT13302.0642576E-57.6185061
CTTCAAG19954.083995E-87.255721
CTATAAT10250.0051739547.0610541
CTTCTTT32403.6379788E-126.70146371
CAAAGAT19651.7878392E-66.6298451
CTTCTAT14000.00233244876.2036411
CTTCCAG19608.14361E-55.90822941
GTTTTAT18452.2771028E-45.8842121
TCAAGAT15950.00159890295.8813593
CTCAAAA15700.0083406965.5319091
CTTCAAT18400.00139668055.5068551
CTTCCGG20107.2505255E-45.40118031
CTTTTTT42901.0732037E-95.2299541
CTTTTAT20850.00118316795.20689251