FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013566

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013566
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1823142
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT5950.033.7866637
ACACGTC6500.032.0323568
CACGTCT6000.031.1118859
GCACACG12550.018.3052126
AGAGCAC11950.016.2205053
GAGCACA13450.015.4803214
AGCACAC13650.014.7263075
GAAGAGC22400.08.6747641
AAGAGCA28951.8189894E-127.19364452
CCTCAAT16356.194563E-67.04277941
CTAATTT10600.0075711376.7894721
CTAAAAT15351.4680726E-46.56389431
ATTTACG12650.0060680216.2426746
CTTCGGT16754.5383268E-46.01526981
GAAGATT30056.618211E-85.73543452
TTTACGA17659.133855E-45.6949197
CTCGTAT8808.54925E-115.384454335-39
CGCTTAA9551.5825208E-105.11188625-29
AATTCAG19700.00364735285.10188065
CCTGAAC19850.00388713485.0758571
Produced by FastQC (version 0.11.5)