FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013573

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013573
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1318483
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTTAG7251.46376115E-510.9269915
CTCCTAT8153.6266483E-610.8156661
ATTTAGG5750.002905820710.0200086
CTCACTT5900.00305354259.960191
CCCAAAT7953.6385667E-49.23979951
CCCCCCC1852.1174269E-48.56342590-94
CCCACAT8607.969541E-48.5414431
GTTTAAT9903.5505227E-48.1618221
CTTCGAT13351.7823819E-57.7032931
GTTGATC9500.00258811457.580642
CTTGAAG15802.7352344E-67.43862341
CTTTAAT18959.854557E-87.36502171
ACCAATC10650.0078694026.7620732
CTTAAAT12250.00341400366.59608461
CAATAAG13650.00148486116.45770551
CTCAAAG12750.00518511336.33741471
CTTATGA13950.00189726046.318831
CAACTTT12750.0063685776.21338753
CTTCAAA23153.1461987E-66.0288191
CCTCAAA14700.0034123715.9964411
Produced by FastQC (version 0.11.5)