FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013575

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013575
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences582806
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC2553.5834091E-927.9777648
AGAGCAC2809.709765E-925.479753
CACGTCT2551.1067459E-725.1799879
CACACGT2752.2958557E-723.3487177
GAGCACA3102.8623617E-823.0139664
GCACACG3153.3915057E-822.6486666
AGCACAC3251.1452339E-619.7566055
GAAGAGC6900.00131420839.3272531
AAGAGCA8000.00510837148.0295662
CTTTCAG14400.00364918265.9590791
AGTCACA3200.00190464945.79664320-24