FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013576

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013576
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences632713
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAGAG2359.18005E-418.5150971
CTTGTGA4300.003665637211.8051691
CTCCCGA4450.00473540811.4072421
CTTTAAT9802.7024726E-811.0996121
CTCTCTT5250.001328691711.0065864
CTTTAAA11201.7245839E-810.3596381
CTCATTC6300.00582847149.2085681
CTTCCGA6600.0085381658.7899961
CTTTCAT12901.4723355E-68.4322641
CTTCTAT10054.7823047E-47.9372351
TAAATGA9100.00165548417.93179669
CTTCAAT11201.7853269E-47.7697281
ATTTAAT11853.4229952E-47.34354071
TCTATTT10900.00118415677.2910692
CCTTCTT10400.0058368226.97283361
CTTCCTG11800.0026329356.76010271
CTTTTAT14103.387224E-46.68600751
ATTCAAA13200.00117273716.5924971
GTTCTTT14554.96545E-46.4792241
CTTTCTT19057.5436546E-66.47137551
Produced by FastQC (version 0.11.5)