FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013577

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013577
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1844436
Sequences flagged as poor quality0
Sequence length100-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT6759.573864E-510.5492857
CTCGAAT9000.00160282527.9596521
CACGTCT8950.00160977657.9561659
ACACGTC10100.0052549527.0502658
GAGCACA13450.00178470046.35361774
ACAAATT13950.00268185866.12738472
AGCACAC13100.0094832145.97927055
CAAACCG17307.280639E-45.79720351
TAAATAT19807.373296E-45.39495943
GTCAAGG22651.290293E-45.3767191
CAAAGAT22908.664947E-45.0051961
Produced by FastQC (version 0.11.5)