FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013581

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013581
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1027302
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATAGG4700.00743330810.737676
TTAACAC4800.00868995810.5139688
CCCAAAT5750.002634438710.1387871
CTTAAAT10305.9740523E-79.90500451
CTCGAAG7502.1950998E-49.7163381
CTCAAAC6809.688544E-49.6448941
CCCGGAT10901.2935998E-69.3597761
CTCTGGA8255.703275E-48.83303451
CTTTAAG9753.280702E-48.2215171
CCAATTC7900.00416825578.2142433
CTCAAAA12659.653371E-68.0649441
TATTGCC10356.9864624E-47.6623547
CTTCGAT13652.6599964E-57.47410631
CTTCAAG14656.761755E-56.96392871
CTTCAAT18053.023848E-66.8633411
CTTCCCG10700.0073414256.81051731
GGAAGTT10750.00767979966.778841
CTTCTAT10750.00767979966.778841
ATTTACC10800.0089070186.6755356
CTTTGAT19358.942141E-66.4022381
Produced by FastQC (version 0.11.5)