FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013597

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013597
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1076254
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATCT6200.00528623869.3195763
CCCCCCC1604.4886093E-49.049112105-109
CCTCTCC8558.6518115E-48.4707941
CTCCAAT8850.00121761368.1836481
CTCAAAG10701.0684125E-48.1224611
CTTCGAT9450.00232384057.6640511
CTTTAAT13400.00141305066.4858461
AATTCAA15802.039268E-46.3992415
CTTAAAA14750.00414701275.8922261
CTTCAAT16050.00164858255.8662221
CATTGAT16100.00171101725.84800431
CAAAGCA15000.004998795.79402261
AAATACT16450.002287275.7073566
CCTTGAA17000.00326720185.5384041
CTTTTAT21653.3566292E-45.35244561
AAACTTT19550.0030703015.17224554
Produced by FastQC (version 0.11.5)