FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013605

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013605
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences865737
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTCT3901.0413336E-414.8841411
CTCGACT3150.006461693413.820991
CTAAAGA6153.058795E-511.7984051
ATTAAGT5852.5174837E-411.1631071
CTTAATA7151.1535831E-511.1631061
CCCAAAA7702.6186577E-510.3657411
CTTAACA5700.002538510.1838871
CTATAAG6800.00100738239.6035551
CCCAAAG6350.00619368449.1414411
CTTTAAT11101.7335491E-58.498041
CATAGAT8200.0055048337.9639241
CTTCGAT10406.864517E-47.6746361
CTTCAAA15302.0256266E-67.5879941
TCGATTT8650.00960461057.4837693
CTCAAAA11953.7493012E-47.2863791
CAAAGAA21107.903145E-76.5338561
CTTTGAT15601.6245415E-46.51181171
ATTGGAA12900.0066463876.1873031
TTGGAAA17507.9879945E-45.75418662
Produced by FastQC (version 0.11.5)