FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013606

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013606
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3487227
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAAAT21554.0563464E-107.67974571
CTCAATT18851.294411E-77.2528471
CTCATGT13301.8299576E-47.0332811
CCCACGT13853.0068136E-46.75398161
CTCAGAT13150.00123139266.56631571
CCCCTGT15551.7429549E-46.47834251
CCCGCTT38050.06.4296971
CTTAAAT20604.2754073E-66.28740641
CAACGAT34401.4733814E-106.0660481
CTCACGG26451.9024446E-75.98498771
CTCATGA16854.914351E-45.978531
CACCCTT21751.0323498E-55.95496941
CTCAAAA37553.6379788E-115.9404321
CTCTTGG14650.00414055355.89399671
CCCAGAA28151.0297481E-75.87916561
CTCCAAT21154.2812262E-55.78368761
CTTCATT45851.8189894E-125.64975261
CTTCGAA26751.4781581E-65.64887241
GTTAATT20752.1294308E-45.52729852
CTTCAAT50800.05.5241721
Produced by FastQC (version 0.11.5)