FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013610

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013610
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1526646
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC5459.226938E-613.2602048
CACACGT5059.5712516E-411.448797
CACGTCT6103.853037E-410.6625089
TAGGAAT10550.00694636676.85005769
CCAGGAT11700.00249173826.7971092
CCAACAT12500.00473298556.39330671
AACTTAT16955.0064444E-45.9692435
CTTCGAT15950.00147515655.9214161
CTGCAGG14750.00400871745.9106071
TTCCGGG21404.755592E-55.7431822
CTAAAAT16600.00237591825.68955331
CTTCCGT20901.9446427E-45.56181531
CTTTGCT18500.0014266975.49792961
GTTGATT21152.3049321E-45.4960731
CAGGAAT25108.688391E-55.2100471
CAACAAC19600.00294886415.18937251
CAGAACA19900.00379942965.08502153
GTTCCGG24553.4811E-45.03083751
GTTTTGA21700.0019134675.0219731
Produced by FastQC (version 0.11.5)