FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013618

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013618
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences764981
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACTA1658.0488535E-526.5370351
CTCATAT6605.9281716E-511.0570981
CTCAAAT6300.00553553749.2669021
CCCTCGG6450.00671692159.0513931
CTTCATT13101.7597267E-78.9132031
GTCAGAA7850.0035285158.3667731
CCTTCAT12508.060359E-68.1734071
CTTCCGA8150.0049476098.0587931
TCAGAAC8200.0058103967.91593462
ATCAAGT8350.00615133867.86576841
CTCATCC8550.00760421257.68177371
CTTAAAA12406.443908E-57.65079931
CAACAAT11502.2547916E-47.61497551
CTTCAAT12405.360971E-47.0622761
CATCAAT15702.1806949E-56.97231051
CTCTTCC10550.00631130356.9172371
CAACTTC10500.00674691846.86975154
CATATCT11850.00257748186.77422241
CAACAAC11950.00281549386.71753451
ATCAAAT14454.2323704E-46.5653911
Produced by FastQC (version 0.11.5)