FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013632

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013632
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences451577
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTTAA3200.00719490213.596391
CCCAGAT6404.6133977E-511.3303241
CTTCAGA4850.00897665710.4659481
CATTCAG4850.00897665710.4659481
ACAATTT6406.032405E-410.153243
CTTCAAT6900.0011557849.4583571
ATCAGGA9251.9528778E-48.6232951
CTTTTAT10155.3120806E-47.8586681
AGAATTT10150.0048021087.11338144
CTTTTTT21504.836337E-55.73367071
AATTTTT15800.0091856965.4817855