FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013642

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013642
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1606276
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACTTG10609.115458E-47.4743314
CTCAACG10000.00411273357.2299961
CTCCGAA15051.3777961E-57.20597551
CTTTTAT21652.5122063E-87.0129291
CTCACGC10350.00574642476.9855031
CTTTAAT12506.5496075E-46.9407961
ACAAATT13802.8398147E-46.78499844
ATAACAT11900.00309754726.65780743
CTTCCAT32401.891749E-106.24814461
CTCAAAG16553.6646466E-46.1160091
CCCGCCG17901.5294431E-46.05865571
CCCGTAT16750.00280075295.611341
CTTCAAA27352.0324296E-65.5513681
CTTTCGG27752.6543257E-65.47134831
TTTGATA24456.443244E-55.30975442
CTTTGAT22004.324728E-45.25817871
CATTTTG19300.0027096695.22463855
GTTTGAT18200.0074427715.16428331