FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013645

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013645
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1200977
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTAAT4650.006277182610.9849551
CTCCACG8305.6195422E-59.670921
CTTAAAG9251.7711242E-59.4665711
CTCCAAT10901.2707023E-69.3724841
CCCGGAA7853.4245662E-49.295731
AAGCGAC7853.819081E-49.19510655
CCTTAAA7400.00207017578.8749091
CTTATGT7400.00207017578.8749091
CTTCTAA8709.5446245E-48.38752651
CTTCCGA8950.00126351648.1532381
CACAGAT12608.995252E-68.1079431
CTCCATT14457.8674566E-78.0798871
CTCAGAT11753.3015847E-58.07344151
CTCAAAA16751.0939584E-77.84171151
CTCCCAA9500.00227381997.68120961
CTTCTAT17402.0732477E-77.54877421
CCCAAAT10900.00106714377.36409431
CTTCAAT21351.6067133E-87.1775231
CCTCAAT13451.7646779E-47.0530061
CTTTCGT11400.00171695327.04110771
Produced by FastQC (version 0.11.5)