FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013648

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013648
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1006321
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTCGC2003.1937758E-421.657476
CACTAAT3900.00167880113.1013251
CTCAACA6955.737784E-712.6031241
CTACCAA4755.2614964E-412.2935731
CTCCCAC5752.0306393E-411.4250061
CTTTCGT8456.0214006E-610.3658831
CTTTAAC6657.7775633E-49.8787641
CTCGAAA8305.5988734E-59.6737831
CTTGGTT6200.0048454719.4184641
CTTGAAC9352.003207E-59.3680971
GTCAAGT6250.00517698339.3431161
CTCCTGA7903.6373475E-49.2396321
CTGTCGC6450.0067077779.0534071
CAACGGA9903.928881E-58.8476481
CTTCGAT10104.7092474E-47.94974231
CTTCTAT12205.255109E-57.77795271
TTCGATA10306.5331784E-47.71054552
CTTCTTA9950.00356560367.3359891
GTTTTAC11400.00192262876.9658536
CTTGTAA10600.0065971796.8861411
Produced by FastQC (version 0.11.5)