FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013649

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013649
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences993706
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAAT3050.00525219214.2602351
TTGATCT7700.003532648.3662163
CCCAGAT7900.00397167878.25831
GTACATT10650.0083431066.7209032
CTTTGAT16906.677501E-56.43398141
CTCAAAA13900.00211350926.25808861
TTTCGTT12750.00678396926.17532352
CTTCAAT17356.4832583E-45.84929941
ATTTAAA18150.00115081045.5914791
ATGATTT24708.511799E-55.21618273
CTTTTCT25108.980071E-55.1984521