FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013650

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013650
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1329255
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAGAT7400.00222739138.8038251
CTCCGAA12408.5958527E-78.7564931
CTCTTAT10458.165567E-58.3123851
CGAATCC9900.00377058057.2944913
CTTTCTA11450.00195804476.95421081
CGCTTTA10550.0069941186.8450674
CTTCATT22403.3677316E-76.46312571
ACTTTCC15057.885361E-46.237885
CTTCGAT14250.00284019656.09574841
CTTTTAC19507.549708E-55.9394471
CTTTTAA22751.1774434E-45.4091391
CTTCTGT17750.00547174835.3015841
CTAAAAA18050.0066594135.2134691
CTTTAAT19650.00318491855.15734431
CTTCAAA24202.9675273E-45.08503771
GTAAAGA20150.0043551475.02937031
Produced by FastQC (version 0.11.5)