FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013655

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013655
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1682091
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGGT5501.5331144E-411.77656653
GTTGGAT9402.382141E-59.2326831
CTCGGAT8150.0053716667.9865541
CATTGGT10250.00521393077.05587151
CCCGAAG15151.1604194E-46.68328431
GTTCTAT14153.6668454E-46.64448641
CCCGCAA16050.00167856165.85791251
CTCAATG15100.0054714075.74749851
CTCCAAA15450.0070498535.61729571
CCTTCAT19705.5952143E-45.5068031
TCGGATT15750.0091985235.48326872
CTTCAAT23804.024175E-55.46978241
CCTGAAG17550.004842255.3572361
GAATACC18450.0092238125.0701566
Produced by FastQC (version 0.11.5)