FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013660

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013660
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences526198
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGCACACGTCTGAACTCCAGTCACAAGTCGAGATCTCGTATGCCGT5460.10376322220913041TruSeq Adapter, Index 15 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT2450.039.831287
CACGTCT2450.039.831289
ACACGTC3350.029.130348
GAGCACA3750.026.0231044
AGCACAC3800.025.6806955
AGAGCAC4153.6379788E-1223.5148533
GCACACG4701.8189894E-1120.7631136
AAGAGCA7204.9112714E-1014.5273432
GAAGAGC6803.266905E-914.4029881
GTTTGAA5900.0045632029.4857441
CCAGTCA3507.024937E-97.98142620-24
CTCGTAT2851.2443197E-67.839758435-39
CACAAGT2704.902373E-67.753518625-29
CAAGTCG2901.6315771E-67.70004625-29
CAGTCAC3053.4354143E-67.327210420-24
TCGTATG3053.4471723E-67.325536340-44
AGTCACA2704.044606E-57.24240520-24
ACTCCAG3104.5033576E-67.195601515-19
AGTCGAG3351.8632909E-67.079758630-34
ACGTCTG3259.09614E-66.863236410-14
Produced by FastQC (version 0.11.5)