FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013689

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013689
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences656841
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT2156.708906E-726.694027
ACACGTC2202.4162775E-522.826428
CACGTCT3052.9692185E-416.464969
AGAGCAC3559.4150525E-414.1437973
GCACACG4200.003332352711.95669656
GAGCACA4958.5982046E-411.5943724
AGCACAC4600.0065684110.9169845
TGAAGGG5600.002407187810.2485978
CAAGAAT10659.726789E-47.42731431
CAGTCAC3303.9025067E-46.08676720-24
Produced by FastQC (version 0.11.5)