FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013691

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013691
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1258493
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTAAT11452.0008883E-1111.8124831
AACTTAC6150.00579587469.2161526
CACACAT6500.0087796988.7613241
CCCACAA10459.923371E-58.1744411
CCCACGA8150.00619058267.8610041
CCCAGAA11753.851441E-47.27003431
CTTTGTT15955.0352373E-67.1408911
CTCTGTT11300.00203249746.9295871
CTCATGG10300.00637149356.91123871
CTCATTT10350.00667742556.8778511
CAACAAT16204.7834204E-56.5912741
ATCTGTC14104.509578E-46.53219276
CTTAAAA12000.00382163126.52536061
GATAATA14154.689719E-46.5111812
CTCCATT14606.497892E-46.33845761
CTCAAAA15802.4594637E-46.30759861
TAAATTT12500.00614777166.23447943
CTTTTAA12700.00689809776.16569571
CTTTCGG16253.5342594E-46.13292651
TTTATTA15300.00120539876.02177862
Produced by FastQC (version 0.11.5)