FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013694

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013694
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1717988
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAAAT12751.3798854E-89.6095641
CTTGCAT14105.740445E-67.6672061
CTTTAGT9400.00231618677.6672061
CTTGTAT17101.5411752E-67.1650261
CTCCAGT11800.00281612746.71855161
CTTGAAT17551.7291572E-56.57064251
CACAAAA27751.6716513E-96.4929491
CCCATTC13350.001433046.4783581
CCTCGAT14655.8343867E-46.39544441
CTTATCT20453.6898018E-66.3437221
GACAATC16453.7412887E-46.10625932
CTACAAT16553.8174356E-46.09670261
GTCCATT13050.0080466286.0750121
GGACAAT15450.0011084776.06428861
CTATTAT25706.8110967E-75.889131
CTCAAAA24902.4235596E-65.7888941
CTTTATT37852.6375346E-105.71242241
CGTTTTG18350.00151626945.4716144
ATTAATC17150.00405414735.4386662
CTTCCAT31107.3584124E-75.3300641
Produced by FastQC (version 0.11.5)