FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013695

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013695
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences930925
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTACT3650.004376047814.65703145
GTACTCG4200.003205523412.0197098
GATCGAT4600.00610597911.0254481
GCTATTA6200.00535328459.3050815
CGATCAT8801.15012794E-49.0566181
CTCCGAC7500.00249370448.6943541
CTTCTGA11854.3361288E-68.5598411
AACATAC7650.00311280838.4840445
CCCTGAT8050.0047260228.1003291
CTCAAAT8200.00557930677.95215231
CCCGGAT11904.225562E-57.91502761
CCCAAAA9200.00177813627.875321
CTCCTGA8450.00730330787.71688181
CATCCAT12407.053258E-57.5958731
GAATTTA12407.470626E-57.5607854
CCACAAT8650.0090017227.5384571
TTAAATT14506.721772E-56.9668842
ATTCAGG10600.0070877846.83518361
CTTCAAC12150.00361592026.55952551
GTATTCC13250.00130566216.53111366