FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013697

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013697
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1336844
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAAACG6300.0062354639.1346463
CTCAAAA17201.821536E-88.10337451
CCCAGAA11853.4376742E-58.0475891
CTTCGCA10305.51311E-47.8342291
CCCAAAA12204.9437804E-57.8167151
CCCGTAT9450.00205041447.7626311
CCCAGTT9500.00215954877.72177551
CCCACAA8750.0089342687.54527761
CCTCAAT10809.148639E-47.47153231
CTCCAAA11903.1551448E-47.3973311
CTCTGTT12650.00484475266.37885761
CTTCAAC16352.5962738E-46.28132151
CTTCAAT22852.5782156E-66.09969571
CCTCCAT14800.0037366525.94785361
CTCCTGT14900.0040277845.9079351
CTTGTTT17455.9989153E-45.8853651
CTTTGGA15200.00502697155.79133131
CTTCTTT41801.0913936E-115.79133131
GAAGATT20151.32495E-45.71241472
CTTTCGG17250.00334002995.52834371
Produced by FastQC (version 0.11.5)