FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013698

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013698
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1124020
Sequences flagged as poor quality0
Sequence length100-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTCT4504.015945E-412.69431
CCCAAAT6708.625389E-510.6575271
CCCGGAT14551.2117725E-67.8521441
CAACATT8500.008772217.56057451
CCCAGCT13452.3028339E-46.901641
GTCTTTC12700.00676347136.1772912
ATCGAAA15350.0076496055.5754762
CCACCGG16850.00347981355.5090241
CAAAGCC19455.587852E-45.50684551
CCGGAAT20752.2440734E-45.5059611