FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013701

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013701
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences894377
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCCTA2550.001645213316.9733434
CTTAGAT3900.001690616813.0888581
CTTCAAT14450.011.1025611
TTACGCT5600.002300871510.3052443
TTTCGTG6759.94504E-49.6171527
CTTTAAT14307.512426E-99.1791991
CTTTGAT14357.9762685E-99.1472161
CTCAATT7200.00162222359.1154551
CAATGGA9001.3685698E-48.91288951
CTTTGTT14408.1994585E-88.609041
CTTCCCG9552.6026E-48.3995821
CTGGACG11302.0380905E-58.3894451
CACGATC7750.0034904528.3771663
CTTCCGA8200.00526349348.0038141
CTTTCAT15101.5418627E-67.7270081
CTTCCAT14305.918524E-67.64933251
ATTTCGT9600.00282201877.51346
CTTTTGG11652.6421665E-47.5114481
TTCAATG12801.1032283E-47.3263842
CTTTCAG14254.6516383E-57.16442781
Produced by FastQC (version 0.11.5)