FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013706

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013706
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2945713
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAGGT12457.504117E-57.55962371
CCCAATT11652.8787146E-47.45729831
CCCACGC17151.5049827E-67.1764721
CCCAGAT26608.0035534E-117.076491
CTCACAG16456.214752E-67.0417441
CTATTCG10500.0065241326.8950421
CCTAAAT13953.0458282E-46.7467611
CTCACTT14003.1818898E-46.72266531
CTCAAAT26353.4106051E-96.59411951
CTCCAAC19802.0223233E-66.58163071
CCCACCG17651.7612338E-56.56298541
CCCAAGG13400.00142150126.48339751
CTCAAAA34903.6379788E-126.43076231
CTCCAAT22704.2835745E-76.3786731
CTCCGAA27357.587005E-96.35301831
CTCGATT16603.747931E-46.10584971
CTTCAAT46300.06.09831431
CCCAGAG13100.0079938726.07921651
CCCAGAA26651.9545041E-75.9765651
CCTCTAT20652.7040916E-55.96012071
Produced by FastQC (version 0.11.5)