FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013707

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013707
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3089978
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAGAG11703.1647316E-58.10199451
CTCAGAC11101.5153934E-47.88302141
CTTGAAT32150.07.71138431
CTTTAAT52450.07.50728131
CCCCAAC11752.9252958E-47.44693951
CCCCAAT12759.224998E-57.4347711
CTCAACG15151.343513E-57.2195991
CTCCGTT25401.7644197E-107.17696331
CTAAGAT16505.845557E-67.07083131
CTCCTGG10350.0052940477.04521231
CCCCCAT12656.815913E-46.91711761
CTCCACT15952.7626284E-56.8574871
CCCAGAT24504.5220077E-96.84535841
CTCCGAG11750.00238356586.8263611
CTCAAAT26956.712071E-106.76418831
CCCAGCT21952.0497464E-76.64400431
CTCCTAT21107.302424E-76.56607061
CTACGAA14504.4725023E-46.53747131
CCCAAGA13400.00131107566.52996541
CTCCAAT23501.0210533E-76.5160721
Produced by FastQC (version 0.11.5)