FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013709

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013709
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1349162
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTTA6003.4532163E-410.7894574
AGACGGC11552.805488E-47.4732182
CTCAAAA15701.9275109E-46.42745731
AATTTTG18253.157123E-56.30616665
AGAACCG13750.00204279556.27750254
CTTTCAT23804.2420725E-55.4513791
CTTTCAA22701.2145738E-45.39801261
TCAATGC19600.00333778055.13802773
CAGAACC18200.007896265.1380273