FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013720

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013720
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences669153
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTCT1800.004750197319.7997675
TGGGCAC3050.005870037714.0221298
CTTTAGA5250.001442327710.8990751
CCCTTCT4600.006716788710.8842661
CTTCAAT11905.0185918E-910.2178821
CTATGAA6356.1277114E-410.1374261
CTCAAAG5700.002858301410.0386211
CAACGAT6050.00467809349.4578741
CTCATAT6150.00535463369.3040881
TATCTAC6250.00629014159.1237335
CTCCCAT6600.0095583878.6697181
CTTAAAA7900.004477338.14843751
CTCTTTA7900.004477338.14843751
CTTCATT14258.596053E-78.0308961
CTTTAAT10751.3022109E-47.98420521
ACCAATA8300.0071812027.73082
CTCCAAA8350.0073624027.70930051
CACCTTT8350.0073624027.70930051
CAATCAT10000.0045593217.15251731
CTTTAAA11800.00304357996.66760061
Produced by FastQC (version 0.11.5)