FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013723

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013723
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1500836
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACTA3250.00794807113.3961481
GAGTTGC7850.00385252138.2868146
CGAACGA7900.00407833058.2346414
CTCAGCG8200.00550811137.96417331
GTTTGAT17552.6325506E-77.4423051
GTTCTGT15001.2492139E-57.25624751
CTCAAGG11100.00137459437.1908751
CCTCGAT13201.5020672E-47.14630371
CTCAAAT11350.00174008547.0324861
CGAAAGG13451.8920156E-47.01347261
CTCCGAA12456.002326E-46.99397331
CTCGAAA11450.00190904186.9710671
CTTTATT29251.2732926E-116.94615031
CTTCTGA18103.381907E-66.81525951
CTTTAAT21351.3499266E-76.7974211
CAAAGCT19551.5776714E-66.68094351
CTTCAAT21952.2338463E-76.6116141
CCTTTAA14504.7408344E-46.5056011
CTTCAAA27055.686161E-96.4380751
CTCAAAG15007.1503024E-46.2887481
Produced by FastQC (version 0.11.5)