FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013728

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013728
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1515602
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAAAG12407.299059E-89.4408691
CTCAGAT7150.00147815139.2097991
CTCCGAG8104.5647233E-49.032931
CTCCTAC6550.00746717638.9363961
CTTCGAG8255.482471E-48.8686961
CTCCCGA8500.00705755977.74706651
CTCAATT9800.0030052467.4659941
CCCATGA12103.9380687E-47.2562051
CCCTCAT10250.0046570717.13821841
CCTCATT13451.7086478E-47.0718781
CTCTTGT12656.549606E-46.94071871
CTCTCCT10550.00616270866.93523551
CAATCGT10700.00706545546.83801271
CCCATAT13008.937079E-46.7538531
CCTCCAA10900.0084497576.71254441
CTCCGCT12100.003128046.65152171
CTTAATT12200.00340991516.5970011
CCCAGCT12500.0043962486.43867351
CTTCGAT16102.2002768E-46.36232571
CTCATTT17054.6089987E-46.0078261
Produced by FastQC (version 0.11.5)