FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013731

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013731
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences623546
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT2202.5465852E-1134.7807927
TCCCTAC2152.7039277E-829.1188079
CACGTCT2305.2046744E-827.2197519
ACACTCT2652.0107836E-723.672151
AGAGCAC3701.0682925E-820.682133
TTCCCTA3057.938015E-720.526378
ACACGTC3251.4596335E-619.2632088
GAGCACA4103.4728146E-818.6643644
CACTCTT3755.703634E-616.7001382
AGCACAC4601.2932651E-716.6356285
GCACACG4852.3613393E-715.778126
AAGAGCA7302.3047889E-510.4852472
CCCGGAT6206.235917E-410.1179341
TTTCCCT9002.0637854E-610.0477857
CTTTCCC7754.4646382E-59.8740496
ACTCTTT7352.8529268E-49.4649213
GAAGAGC8258.667294E-59.293511
TTGGATC2501.534683E-57.791397630-34
CAAAAGC10259.017713E-47.4801421
AGTCACT2303.8926647E-47.264192620-24
Produced by FastQC (version 0.11.5)