FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013742

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013742
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences503127
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGG400.007779459518.181103120-124
CACAGAA6354.2985812E-511.4097131
CTCATAT4600.00610015611.0252551
CTTCTCT6951.07625856E-410.4247021
TTTCAAC6254.8160652E-410.4050218
CTTTAAT6708.903118E-49.7323151
CTTATCA6350.0062627329.127771
CTCCTGC7850.00376173048.3065611
CATCAAT10306.2771543E-47.7375581
TCTATTT8700.0096843797.47561453
CTCTTTT12450.0046562116.4013531
Produced by FastQC (version 0.11.5)