FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013746

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013746
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1196868
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTTAC7050.00162250429.115826
CACGACG7800.00405637048.2392996
CTCATTT10651.1172434E-48.0915221
CTTTAAG10508.499211E-47.52318951
ATCGAAT8600.0092568367.51523641
CTTCCGA10800.00114709867.3142121
CTCCGGT12406.4517575E-46.94957351
CTTTTGA19853.2911885E-76.8737181
GTTTCCT13602.461073E-46.8644071
TTCAAAC14608.434643E-56.8478432
CTCAAGA10650.0080864036.7429351
CTAAAAA17301.4742151E-56.6415971
CTTGGAA14103.8255163E-46.6209891
TTTAGCA11950.00354086756.5730538
CCTGCAT14756.6127407E-46.32921651
CGAAGAT14756.6127407E-46.32921651
CCTGAAC13950.0024462496.17739871
CTTCAAA21751.0626492E-55.9430841
AATATAG15700.00150053145.9127035
CTTTGAC15000.005494375.74498131