FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013750

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013750
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5675279
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTCGA16401.2882083E-88.264591
CCCACAC24650.08.10312751
CTCGCGT11101.9544362E-47.71205041
CCCACCT21504.638423E-107.63134241
CCCCTAT20007.1013346E-97.4903291
CTCAGAT28051.8189894E-127.37524941
CCCACGT12801.2703091E-47.24510961
CTATAAT67800.07.154691
CCCTTCT45200.07.1020821
CCCTCGA19352.3810026E-77.00461341
CCCAGAT44100.06.955711
CACACGA19653.1125273E-76.89767361
CTTCGAA41400.06.7201021
CTTCGAT52550.06.6517351
CTCACGT16154.4621644E-56.62567761
CTAAAAC36000.06.5391761
CTCCATT74150.06.44577651
CTCATTT66550.06.43153761
CTCCGCT45550.06.4210651
CCTACAC22856.29896E-76.24389171
Produced by FastQC (version 0.11.5)