FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013754

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013754
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences831413
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGACAC4500.0010183913.997747145
GGTTAAG3150.006827283713.7065116
CTATCCT4850.008331181510.5735041
AGATACG6850.00116229539.4544916
CGAAGAT8851.0839974E-49.1056731
CCCATTT9853.5443383E-58.9249891
CTTCAGG9151.557669E-48.8071271
CTCAAAT7550.00239502138.7328941
CTTATAT12557.133884E-57.588611
AAGATAC10200.00521488257.0548225
CGAAAGA10800.0076277246.78326651
CTCTTTT19901.7988386E-66.62646771
CATAAAT14704.976255E-46.47871161
CAAAGAA21104.6896803E-66.24960661
CTTCCAT15308.077587E-46.22464471
CTTCAAA15308.077587E-46.22464471
ATCAATG14350.00268246126.1262111
CTTTCAT14700.0035118315.9803491
CAAAAAG22901.7627965E-55.7583711
ATTTCTG16400.0023009315.70408156
Produced by FastQC (version 0.11.5)