FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013756

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013756
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3100361
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT9901.4551915E-1113.1028759
CACACGT9452.64748E-59.1510677
ACACGTC12357.188583E-57.5858758
CTTAATT15158.2459656E-46.2155411
GCACACG19558.3748164E-55.8978746
AGCACAC19951.12190406E-45.77962065
CCCTCAT18850.00187868155.37978461
GTCAAAT23001.3770831E-45.3538881
CTCAAAT23101.469776E-45.3307111
AATTTAC23702.3291225E-45.16919375
Produced by FastQC (version 0.11.5)