FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013758

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013758
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences767434
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGCACACGTCTGAACTCCAGTCACCGAACTGTATCTCGTATGCCGT17410.2268598993529085TruSeq Adapter, Index 22 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT5200.051.8491487
CACGTCT6550.043.279239
ACACGTC6950.042.778028
AGAGCAC7350.041.3852653
GAGCACA7650.037.0512434
GCACACG7900.035.0036476
AGCACAC8250.034.3566065
AAGAGCA12650.023.5163362
GAAGAGC13650.021.3731041
CTAAGAA3152.431611E-517.641291
TGCCTAC4400.00621130810.9997389
AGTCACC6300.010.3415120-24
TCTCGTA6600.010.27713735-39
CAGTCAC6300.010.12147820-24
ACTCCAG7050.010.00210615-19
CTCGTAT6400.09.7331335-39
TCGTATG7400.09.54246340-44
ACGTCTG7200.09.41088710-14
AACTGTA7800.09.22267430-34
CCAGTCA7800.09.06363220-24
Produced by FastQC (version 0.11.5)