FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013763

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013763
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences889533
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAGTT4000.00209622312.7199141
CTCAAGG5752.1114277E-411.3768181
CCCAAGT4550.00549602611.1823421
GCCAGGA4650.006461174710.9418611
CTAAGAA4700.00699578110.8254581
CTCATAT7051.2062269E-410.309961
CCCAAAT6506.5552356E-410.0641081
CTATGAG5800.002890934710.0255481
CCCAGAT7000.00129268459.3452431
CTCCAGA7100.00147132679.2136191
CTTAAAT8500.0074811767.69608261
CTTTGAA16608.404295E-77.4436671
CTTCAAT14951.1614027E-57.2928321
CTTTAAT12003.8568416E-47.26852231
CAACAAT11050.00128555447.2356331
CAAAAGG11300.00162941457.0755521
CTTTCGG12405.6153035E-47.0340541
CCAAAAT12200.00364991566.55358551
ATCAAAT14000.0022217186.2301621
CTTTTAA13050.0073609256.1267241
Produced by FastQC (version 0.11.5)