FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013770

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013770
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences964651
Sequences flagged as poor quality0
Sequence length100-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCATCTCGTATGCCGT10420.1080183403116775TruSeq Adapter, Index 18 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT4050.036.2184037
ACACGTC4650.031.545068
CACGTCT5200.028.2085639
GAGCACA7900.020.3370654
GCACACG7250.020.2323476
AGCACAC7650.019.1744485
AGAGCAC8500.018.0787663
AAGAGCA12450.015.7173352
GAAGAGC13300.013.6960211
GCTCCCT4400.00575928611.1124635
CTTAAAA7803.7758728E-610.7785251
TCTTACC7350.00289305658.5530347
AGTCACG4001.5643309E-108.03222520-24
GTCACGT5259.767973E-106.65194620-24
CTCGTAT5052.9504008E-96.63925535-39
TCTCGTA6257.530616E-95.811561635-39
AAAAAAA24250.05.5880460-64
ACTCCAG5601.0196654E-65.48774115-19
CTGAACT8151.2551027E-105.48496510-14
CAGTCAC6006.733899E-75.35481620-24
Produced by FastQC (version 0.11.5)