FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013772

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013772
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1848013
Sequences flagged as poor quality0
Sequence length100-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGAT15352.7861097E-77.97980071
CTCCAAT13201.9841598E-57.64197541
CTCCCAT14450.00349702925.98363261
AATAACC14400.00351309945.9811787
CCAATCC15050.00567575035.7285893
CTTAAAA23503.4910823E-55.5189461
TCAATCC17000.00359706955.49411063
GTTTGCA17350.00442216645.3987771
GATCGAA19000.0023254195.2889065
CTTCGGA19200.00252757785.25385761
CCACGAT21200.00156861145.09808351
GTTCCAG32551.823877E-65.0912961
CTGTGAT18500.0093742815.06317761
Produced by FastQC (version 0.11.5)