FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013773

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013773
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1407518
Sequences flagged as poor quality0
Sequence length100-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCTGA12055.345646E-89.6332921
CTCACTT6800.00100942749.6023031
CTTCTAT20750.09.4403361
TCATGAG7753.2953324E-49.3317452
CTAATTA8155.2811287E-48.901931
CATCGAT10801.2314233E-58.7329581
CTCAGAA7700.0031284348.4799551
CTCCGTT14801.3881981E-78.333531
CTTAAAG11352.3009841E-58.3097751
CTACCTT11552.8643766E-58.1658831
CCAGTGC9150.00172312037.9011273
CTTACAT9200.00175605567.8859491
CATTCAT19653.619789E-97.75351331
CTTCCTC21553.3833203E-107.7432331
CTTTCTA15001.5103178E-67.73874471
CTCAATT11251.874797E-47.73874431
CTATCAT15402.2434688E-67.5377381
CTTCTCA11552.5409998E-47.5377381
CTTTCTT44450.07.5080621
AGATCTT9750.00321370157.4149044
Produced by FastQC (version 0.11.5)