FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013787

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013787
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences681031
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT2155.273064E-420.1055167
ACACGTC2608.472681E-519.3966678
CACGTCT2700.00243639416.0040729
ATCTTAC4958.29881E-411.6435996
CTCTGAT5802.0958002E-411.3850691
CAAGCGT4550.005122705411.2877621
CTTTAAG6053.0993205E-410.9146131
AGCACAC4700.007468114610.7300725
TGATTTC15850.0097230645.45449354