FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013800

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013800
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences815214
Sequences flagged as poor quality0
Sequence length100-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTCGA2000.007387568218.3429581
CCCAGAC4550.00512348111.2879751
AAACTAC5750.002902940810.0203625
CCCAGAA6859.7280205E-49.6400961
CTTCAAT15651.4649164E-46.5636151
CAAAGCA14150.00225249686.22234631
TCTCCAT12900.0071795316.1413567
CAAACAA13300.0081256826.06834751
ATTTATG14400.00337436686.00177966
Produced by FastQC (version 0.11.5)