FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013809

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013809
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1410171
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAGAG6958.737279E-611.4471851
CCCAAAT8809.4111056E-710.6844341
CTCACAT6000.0039898539.6433861
CTTATAT13352.703855E-78.6682131
CTAAAAG7750.00341152918.3990781
CTCAAAG13502.9414114E-68.0361551
ATTAGAA10155.4838194E-47.838221
CTCCAAT12105.3237367E-57.77049731
CTCCTTA10407.1146974E-47.64980131
TTAAACT9450.00242079987.63256032
CTCAGGA8550.0082477817.6131991
ATTAAAC9600.00275009927.5338951
CCCAGAT14851.1353199E-57.30559541
CTCAAGA10900.00117331527.29889251
TCGATCT12851.1641598E-47.2954113
CTCATTT12901.1776463E-47.2886061
CATCGAT13251.6380867E-47.0960771
CTCAAAT12456.230091E-46.97112271
ATCAATT19752.6654197E-76.95788571
CCTCTGT10400.00599979756.9543651
Produced by FastQC (version 0.11.5)