FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013813

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013813
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2192696
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCAG14756.159375E-46.36712275
CCCGCCT15308.9961156E-46.1706041
CTCAGAT13250.0087059146.02910141
CTCATTC15700.0080418955.55082271
CTCATAT17250.0037649435.47305771
CCCACGG17400.0041698965.4258761
CTTTCAC19150.00221655775.30927231
CCAACGG17800.0054481925.30394651
Produced by FastQC (version 0.11.5)