FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013818

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013818
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1344002
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAA11153.1297805E-68.7700451
CTCAAAT10600.00126315757.2482881
CTCCAGT9700.0042773797.20073651
CTTGCAA14951.5356095E-46.5408691
CATCAGA14055.136019E-46.46272471
CTTGTAT13500.00231227236.2086351
CTTCCAC18455.6791127E-56.05720471
CTTTTGG14150.0039121455.92343241
CTTCCAA18203.06947E-45.7566331
CTTCGAT15000.0074689455.58777141
CTTTGAT21558.912292E-55.50998351
CTTGGAA16800.0043613365.4048381
CTTGAAG19708.92343E-45.31831031
AACGAAA17600.0077526745.146116
Produced by FastQC (version 0.11.5)