FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013821

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013821
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences464844
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAT1650.002635557221.9165883
CCCAGTT2650.002054712516.416061
CTCCGCT3558.6730946E-414.2966161
TGTCCCG3200.007326136313.5594298
CTTTGAT7006.7629844E-712.4293021
CTTCTCG4500.005150283711.27843951
TCACAAT4600.00618405211.0047542
CTTTTAA7552.4629824E-49.6032141
CTTCAAT9552.7738066E-59.1104831
AGAATGG6600.0087267828.7656916
CTTTTAT10200.00483643127.1082611
CTTTCTT15000.00492199745.8003411
Produced by FastQC (version 0.11.5)